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. 2011 Dec 22;158(2):679–692. doi: 10.1104/pp.111.189704

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Figure 8. — Starch-modifying enzyme activities. A, Total soluble endosperm extracts. Proteins in soluble extracts from 20-DAP endosperm tissue of the indicated genotype were subjected to zymogram analysis as in Figure 7. Band I is the ISA1 homomer, and band II is ISA1/ISA2 heteromeric enzyme. B, Purified amyloplast soluble fractions. Amyloplasts were isolated from endosperm of the indicated genotype harvested 15 to 16 DAP. Mutations affecting various starch biosynthetic enzymes were used as negative controls. The affected enzymes were GBSS (wx^-), ZPU1 (zpu1^-), SBEIIb (ae^-), or SSIIa (su2^-). In this analysis, the second form of ISA1/ISA2 heteromer is evident in band III.