Table I. Cell wall sugar composition of leaf blades and epidermal peels from csld1 mutant and nonmutant plants.
Alcohol-insoluble residues were prepared from both whole-leaf blade sections and isolated epidermal strips of greenhouse-grown plants identified as mutant or wild type by PCR of a segregating family. Analysis of noncellulosic cell wall sugars was done at the Complex Carbohydrate Research Center at the University of Georgia. Values for noncellulosic sugars are given as mol %, with se in parentheses. Cellulose content was estimated based on remaining weight after the hydrolysis of hemicelluloses and lignin. Cellulose content is given as weight percentage of alcohol-insoluble cell wall fraction, with se in parentheses. None of the differences between csld1 mutant and wild-type samples were statistically significant at P < 0.05 (n = 4 for each sample). nd, Not detected; nt, not tested.
Sample | Plant | Ara | Rha | Fuc | Xyl | Man | GalA | Gal | Glc | GlcA | Cellulose |
Whole leaf | Wild type | 11.43 (0.33) | 0.53 (0.02) | nd | 81.40 (0.01) | nd | 1.36 (0.07) | 0.85 (0.69) | 4.43 (0.41) | nd | 26.6 (1.8) |
csld1 | 11.84 (0.18) | 0.57 (0.05) | nd | 81.18 (1.31) | nd | 1.59 (0.016) | 0.80 (0.04) | 4.03 (1.41) | nd | 22.9 (3.1) | |
Epidermal peels | Wild type | 10.66 (0.54) | 0.31 (0.02) | nd | 85.04 (0.69) | nd | 0.95 (0.29) | 0.65 (0.08) | 2.39 (0.34) | nd | nt |
csld1 | 11.93 (0.63) | 0.34 (0.03) | nd | 84.37 (0.96) | nd | 0.71 (0.05) | 0.69 (0.08) | 1.97 (0.22) | nd | nt |