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. 2011 Dec 20;158(2):737–746. doi: 10.1104/pp.111.190223

Figure 4.

Figure 4.

Activity of Arabidopsis CSD1 variants in yeast sod1Δ and ccsΔ. A, Sequence alignment of CuZnSOD in Arabidopsis (CSD1, CSD2, and CSD3), human (hSOD1), S. cerevisiae (ySOD1), and C. elegans (wSod-1). Residues corresponding to 141G and 143V of CSD1 are indicated in white boxes with arrows. Identical residues are shown in black. B, Yeast sod1Δ and ccsΔ expressing the CSD1 variants were analyzed as described in Figure 1A for SOD activity (top) and CSD1 protein level (bottom). C and D, Viability of yeast sod1Δ and ccsΔ expressing the CSD1 variants under Lys-lacking conditions. Plate (C) and liquid (D) assays were as described in Figure 1, B and C. The data are from four independent tests (means ± sd). WT, Wild type.