Figure 6.

Differential complementation of the E. coli grpE mutant strain with Arabidopsis Mge1 and Mge2. Heat-sensitive E. coli mutant DA16 grpE was transformed with Arabidopsis Mges encoding long- and short-form Mge proteins with 38 and 58 putative mTPs removed from the N terminus, respectively. DA16 transformed with short Mge1, long Mge1, short Mge2, and long Mge2 are labeled 1S, 1L, 2S, and 2L, respectively. DA16 transformed with empty vector (V) was used as a negative control. The wild-type strain DA15 (WT) with an isogenic background to DA16 was transformed with the same empty vector to confer the same antibiotic resistance as the others and included as a comparison. Cell cultures were serially diluted and spotted onto LB plates and incubated overnight at 30°C, 40°C, 43°C, or 45°C.