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MiRNA-derived ta-siRNAs in B. napus. A, Expression abundance of ta-siRNAs generated from TAS3 gene TC65909. The locations of two miR390 target sites and TAS3 5′ D7(+) are labeled by short bars. B and C, Quantitative RT-PCR (B) and northern-blot hybridization (C) analyses of TAS3 5′ D7(+) during early silique development. D, Validation of cleavage on B. napus TC110025 (ARF3) mRNAs by TAS3 5′ D7(+) using 5′-RLM-RACE. Error bars indicate the sd of three replicates.
