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. Author manuscript; available in PMC: 2013 Feb 1.
Published in final edited form as: Clin Cancer Res. 2012 Feb 1;18(3):726–736. doi: 10.1158/1078-0432.CCR-11-2521

Figure 1.

Figure 1

MDA PCa 118b prostate cancer cells have an activated Wnt canonical signaling pathway. A, beta-catenin immunohistochemical staining of subcutaneous xenografts with anti–beta-catenin antibody showing strong nuclear and cytoplasmic staining on MDA PCa 118b, strong membranous staining on MDA PCa 2b, and diffuse staining on PC-3 cells. Original magnification, ×400 and ×800 on left and right panels, respectively. B, photomicrographs of those same cell lines immunostained with anti–beta-catenin antibody and visualized on confocal microscopy. Original magnification, ×630. C, transcriptional activation of TCF reporter in MDA PCa 118b, MDA PCa 2b, and PC-3 prostate cancer cells. Cells were transfected with the TOP-flash reporter or the FOP-flash control construct. Renilla was used as a co-reporter vector to normalize transfection efficiency. Reporter assays were performed with a luciferase reporter system. *P < 0.001 vs. MDA PCa 118b cells transfected with the FOP-flash control construct.