MDA PCa 118b prostate cancer cells have an activated Wnt canonical signaling pathway. A, beta-catenin immunohistochemical staining of subcutaneous xenografts with anti–beta-catenin antibody showing strong nuclear and cytoplasmic staining on MDA PCa 118b, strong membranous staining on MDA PCa 2b, and diffuse staining on PC-3 cells. Original magnification, ×400 and ×800 on left and right panels, respectively. B, photomicrographs of those same cell lines immunostained with anti–beta-catenin antibody and visualized on confocal microscopy. Original magnification, ×630. C, transcriptional activation of TCF reporter in MDA PCa 118b, MDA PCa 2b, and PC-3 prostate cancer cells. Cells were transfected with the TOP-flash reporter or the FOP-flash control construct. Renilla was used as a co-reporter vector to normalize transfection efficiency. Reporter assays were performed with a luciferase reporter system. *P < 0.001 vs. MDA PCa 118b cells transfected with the FOP-flash control construct.