Fig. 1.

High-power light photomicrographs of representative interactions between vasoactive intestinal polypeptide (VIP) and gonadotropin-releasing hormone (GnRH) neurones in both (A) prepubertal and (B) adult female rats (section thickness=30 μm). Confocal microscopy photomicrographs of 0.5 μm optical sections of representative interactions between VIP and GnRH neurones in both (C) prepubertal and (D) adult female rats. Red (GnRH) and Green (VIP) channels are separated in the confocal image (and shown together) to clearly indicate the extent of VIP fibres on the same 0.5 μm plane as the GnRH cell body. Green=VIP; Red=GnRH in all images.