Figure 7.

Diurnal and circadian rhythms of GFP-IR and PER1-IR in TH amacrine cells. Retinas were harvested at ZT 2, 6, 10, 14, 18, and 22. For GFP only, an identical series was obtained at CT 2, 6, 10, 14, 18, and 22. Two mice were used per time point, and the values were averaged. For the ZT series one retina/mouse was reacted for GFP-IR and the other for PER1-IR. Then 100 TH cells/retina were examined for GFP (left) or PER1-IR (right). No PER1-IR was detected at ZT 18 or ZT 22. Single asterisks indicate 95% confidence level; double asterisks indicate 99% confidence level for a significant difference between the indicated pairs.