Table 1.
Strain genotypes and products
| Strain | Base strain | Genotype and [plasmid] | Source | Produces |
| Gen 1.0 | ||||
| EPY330 | S288C | MATα his3Δ1 leu2Δ0 PGAL1-tHMG1 ∷ δ1 PGAL1-UPC2-1 ∷ δ2 erg9 ∷ PMET3-ERG9 ∷ HIS3 PGAL1-ERG20 ∷ δ3 PGAL1-tHMG1 ∷ δ4 [pESC-leu2d-ADS/AMO/CPR] | (15) | AA |
| Y87 | S288C | EPY330 gal1Δ∷K. lactis URA3 lys2Δ0 | This work | AA |
| Y135 | CEN.PK2 | MATα erg9 ∷ Kanr-PMET3-ERG9 trp1 ∷ TRP1-PGAL1-ERG20 his3 ∷ HIS-PGAL1-tHMG1 leu2 ∷ HIS-PGAL1-tHMG1 ura3 ∷ URA3-PGAL1-upc2-1 [pAM322: 2μ-leu2d PGAL1-ADSPGAL10-CYP71AV1 PGAL1-AaCPR (codon opt)] | This work | AA |
| Y137 | S288C | As Y87, but plasmid is pAM322 | This work | AA |
| Y151 | CEN.PK2 | As Y135, but plasmid is pAM426 [2μ-leu2d PGAL1-ADS (codon opt)] | This work | Amorph |
| Gen 2.0 | ||||
| Y224 | CEN.PK2 |  , leu2-3,     [pAM322] |
This work | AA |
| Y227 | CEN.PK2 | As Y224, but plasmid is pAM426 | This work | Amorph |
| Y293 | CEN.PK2 | Y227 gal80Δ∷natr | This work | Amorph |
| Y337 | CEN.PK2 | Y227 gal1Δ gal7Δ gal10Δ∷HphA | This work | Amorph |
Amorph, amorpha-4,11-diene; AA, artemisinic acid. A complete list of strains, including progenitor strains, is listed in Table S1.