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. 2012 Jan 3;109(3):989–993. doi: 10.1073/pnas.1115858109

Fig. 3.

Fig. 3.

Structural analysis of the arabinofuranosidase-sensitive xylan oligosaccharide derived from TaXAT2 gux. (A and B) Extracted ion chromatogram (EIC) of the sodiated molecule DP5 after xylanase digest. Capillary NP-HPLC-MALDI-ToF-MS of AX oligosaccharide labeled with stable isotopes of aniline. The EICs of pentose DP5 labeled with 13C6-aniline (m/z 784) corresponds to TaXAT2 gux (blue) in comparison with labeling with 12C6-aniline (m/z 778; red) of (A) gux and (B) TaXAT2 gux after arabinofuranosidase digest (TaXAT2 gux +Arafase). (C) NP-HPLC-MALDI-ToF/ToF-MS/MS of the DP5 pentose XA3XX labeled with 2-AA (see Fig. S3 and ref. 25 for nomenclature). Ions mentioned in the text are highlighted in color. Note: G3, D2, and W2 ions show a α-(1,3)-linked pentose on the penultimate xylosyl residue.