Fig. 4.

CDX1 promoter bisulfite sequencing. Sequencing data, together with CDX1 mRNA expression status, are shown for C70, LS174T, C10, Bristol 8, normal colonic mucosa, and CC07. For each case, relative location and methylation status of CDX1 promoter CGIs for 10 clones are shown. Each circle represents a CGI, and base positions relative to the CDX1 transcription start site are shown above each cluster of clones. Methylated CGIs are highlighted in gray. The horizontal solid lines encompass CGIs within the 200-bp length 5′-flanking region, which shows close homology to its mouse counterpart (see also Fig. 2). CGIs enclosed by the boxes (positions –53 to –65) represent the binding site of the methylation-specific forward primers (see also Fig. 1).