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. 2004 Jan 2;101(2):574–579. doi: 10.1073/pnas.0307190101

Fig. 5.

Fig. 5.

Promoter bisulfite sequencing (presented as in Fig. 4) (A) and CDX1 RT-PCR (B) of LOVO harvested after 4 (D4) and 17 (D17) days' growth. The gel shows increased CDX1 mRNA expression with postconfluent growth. β-Actin loading controls, normal colonic mucosa serving as a positive control (+), water blanks (–), and 100-bp ladders (the Upper and Lower bright bands represent 1 kb and 500 bp, respectively) are also shown.