Table 1. Primers, amplicon size, and specific protocol details for PCRs used.

			Protocol details
PCR	Forward (F) and reverse (R) primers (5′ to 3′)	Size, bp	T°	Time, s	No. of cycles
CDX1 bisulfite sequencing	F:TTGTGTGAAGTTGGTTTAGAATTT	505	56	45	35
	R:ACACATAACCCACATACATAATAAC
CDX1 methylated	F:GTTGGGGTCGCGGGCGGTTC	185	63	45	35
	R:AATCCTTATCCAACACATAACCCACATA
CDX1 unmethylated	F:TTTGGTTGGGGTTGTGGGTGGTTT	189	63	45	35
	R:AATCCTTATCCAACACATAACCCACATA
CDX1 promoter sequencing	F:GAAGGACAAGGTGTTCAGGCCG	421	62	45	35
	R:GTCCAGCACATAGCCCACATACATG
CDX1 exon 1a sequencing	F:CGAGTTCAGGTGAGCGGTTGC	250	60	30	35
	R:CCGGCTCCACGTGAGAGTAGC
CDX1 exon 1b sequencing	F:CCGCAGTACCCCGACTTCTCCA	350	60	30	35
	R:GGCGAGGGAAGGGTCCTTAC
CDX1 exon 2 WAVE	F:CACCTTCCTTCCCGGCTCCTTCTG	222	65	30	35
	R:GTCCTCGCACTGCTCCTATGGC
CDX1 exon 3 WAVE	F:GCTGCCCACTCCATCTCTGTCC	450	55	60	35
	R:CCCAGCACCCGAGTCCCCAG
CDX1 RT-PCR	F:AGGACAAGTACCGCGTGGTCTA	553	66	60	30
	R:CCTCTGAACGTATGGAGGAGGA
Beta-actin RT-PCR	F:ACACCTTCTACAATGAGC	600	56	60	25
	R:ACGTCACACTTCATGATG

T°, annealing temperature.