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. 2012 Jan 4;109(3):905–910. doi: 10.1073/pnas.1108352109

Fig. 3.

Fig. 3.

Cell-intrinsic defects of T cells lacking Smad2 and Smad3. Bone marrow cells isolated from WT (CD45.1+) and CD4:DKO (CD45.2+) mice were mixed at a 1:1 ratio. Cell mixtures were transferred into sublethally irradiated Rag1−/− mice. Eight weeks after transfer, T cells from reconstituted, mixed bone marrow chimeric mice were analyzed. (A) Distributions of CD4 T, CD8 T, and CD4−CD8− cells generated from the bone marrow cells of WT (CD45.1+) and CD4:DKO (CD45.2+) mice were determined by flow cytometric analysis. (B) Foxp3-expressing Treg cells among CD4 single-positive T-cell populations in the thymus, PLNs, and spleens were detected by flow cytometric analysis. The cells originating from WT (CD45.1+) and CD4:DKO (CD45.2+) mice were distinguished by congenic markers CD45.1 and CD45.2, respectively. (C) Lymphocytes were isolated from the PLNs and spleens of mixed bone marrow chimeric mice. CD62L and CD44 expression on CD4 T cells originating from WT (CD45.1+) and CD4:DKO (CD45.2+) mice was assessed by flow cytometric analysis. (D) Lymphocytes were isolated from the PLNs and spleens of mixed bone marrow chimeric mice. IFN-γ, IL-10, IL-4, and IL-17A production by CD4 T cells originating from WT (CD45.1+) and CD4:DKO (CD45.2+) mice was assessed by flow cytometric analysis. Results shown are representative of at least three experiments.