Fig. 2.

miR-122 stabilizes synthetic RNA containing the HCV 5′ UTR. (A) Structure of DNVR2 and PV RNAs (19), which differ only in 5′ UTR sequence. (B) miR-122 slows decay of DNVR2 RNA in HeLa S10 lysate. Data shown represent acid-precipitable α-[³²P]-CTP-labeled RNA in HeLa S10 reaction mixtures (19) containing 1 μM of duplex miRNA. The DNVR2 decay constant in miR-122– vs. miR-124–supplemented mixtures, estimated by fitting the data to a one-phase decay model (R² = 0.972–0.989), differed significantly (P = 0.002). (C) RNA extracted from HeLa S10 reaction mixtures and fractionated by electrophoresis in 0.8% agarose.