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. 2011 Dec 30;109(3):793-798. doi: 10.1073/pnas.1117220108

Table 1.

Steady-state SPR measurements of talin-1 F2F3 subdomain binding to phospholipids

Lipid, % Kd, μM Maximum binding, RU
PS (20%) 0.92 ± 0.260 1,210 ± 66
PtdIns(4,5)P2 (10%) 0.67 ± 0.07 2,490 ± 225
PtdIns(3,4)P2 (10%) 0.42 ± 0.09 2,170 ± 146
PtdIns(3,5)P2 (10%) 0.50 ± 0.053 2,330 ± 193
PtdIns(5)P (10%) 0.37 ± 0.029 1,770 ± 146

Binding to the indicated phospholipids was fit to a one-site binding model using BIAevaluation. The data shown represent the mean and standard deviation of triplicate injections.