Table 1.
Lipid, % | Kd, μM | Maximum binding, RU |
PS (20%) | 0.92 ± 0.260 | 1,210 ± 66 |
PtdIns(4,5)P2 (10%) | 0.67 ± 0.07 | 2,490 ± 225 |
PtdIns(3,4)P2 (10%) | 0.42 ± 0.09 | 2,170 ± 146 |
PtdIns(3,5)P2 (10%) | 0.50 ± 0.053 | 2,330 ± 193 |
PtdIns(5)P (10%) | 0.37 ± 0.029 | 1,770 ± 146 |
Binding to the indicated phospholipids was fit to a one-site binding model using BIAevaluation. The data shown represent the mean and standard deviation of triplicate injections.