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. 2011 Dec 27;109(3):799-804. doi: 10.1073/pnas.1119057109

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Fig. 3. — (A) SAXS curves for 75 μM dT₄₀ (circles) and rU₄₀ (triangles) in 100 mM NaCl are shown together with a WLC model for the scattering (solid lines) that optimizes the fit to SAXS data and smFRET data ( for both rU and dT). Experimental uncertainties were used as weights in the fit. The best-fit values of L and l_p predict values of 68.0 Å for dT and 67.2 Å for rU. The WLC scattering curves include an overall scale factor and an additional free parameter, R_cs, to correct for finite molecular cross-section (see Materials and Methods) equal to 4.2 Å for rU and 3.7 Å for dT. In the inset (B), the persistence length l_p and the contour length L of each WLC are shown as symbols with ellipses that enclose the 95% confidence interval. The nonoverlapping ellipses show that dT₄₀ and rU₄₀ are described by WLCs with significantly different properties: dT₄₀ has a longer L and a shorter l_p than rU₄₀. The cartoon depictions of ssDNA (C) and ssRNA (D) illustrate this difference: the RNA chain is drawn with a shorter distance between monomers and a less flexible backbone.

Inline graphic — (A) SAXS curves for 75 μM dT₄₀ (circles) and rU₄₀ (triangles) in 100 mM NaCl are shown together with a WLC model for the scattering (solid lines) that optimizes the fit to SAXS data and smFRET data ( for both rU and dT). Experimental uncertainties were used as weights in the fit. The best-fit values of L and l_p predict values of 68.0 Å for dT and 67.2 Å for rU. The WLC scattering curves include an overall scale factor and an additional free parameter, R_cs, to correct for finite molecular cross-section (see Materials and Methods) equal to 4.2 Å for rU and 3.7 Å for dT. In the inset (B), the persistence length l_p and the contour length L of each WLC are shown as symbols with ellipses that enclose the 95% confidence interval. The nonoverlapping ellipses show that dT₄₀ and rU₄₀ are described by WLCs with significantly different properties: dT₄₀ has a longer L and a shorter l_p than rU₄₀. The cartoon depictions of ssDNA (C) and ssRNA (D) illustrate this difference: the RNA chain is drawn with a shorter distance between monomers and a less flexible backbone.