Computed free energy of H+/Na+ selectivity of the c subunit binding sites in M. acetivorans, relative to that of the I. tartaricus c11 ring. More negative values indicate greater selectivity for H+ and against Na+. All values are given in reference to analogous calculations for the complete c11 ring from I. tartaricus, previously reported (32). Selectivity values are shown for two alternative models of a M. acetivorans c4 oligomer, one based on the architecture of the I. tartaricus c11 ring (Left), and another based on the c15 ring from S. platensis (Right). The computed selectivity of analogous c4 constructs from I. tartaricus, S. platensis, and B. pseudofirmus OF4 is also given.