Figure 8. Flow cytometry analysis of ROS and RNS production.

A549 cells were seeded at 3×10⁴ cells/cm² and cultured for 24 h. Then, cells were exposed to trypacidin (green area) 10 µM and 50 µM or DMSO 1% (red line) for 1 h, 2 h and 24 h. H₂O₂ production was assessed using H2-DCF-DA, O^2•− using DHE, NO using DAF-FM. Menadione was used as control for H2-DCF-DA and DHE, SNAP for DAF-FM (blue line). Peaks are representative of three independent experiments.