Table 1. Comparison of LC-MS analysis of the fractions obtained from flash chromatography and their effect on the A549 cell viability.

	Fraction
	control	F12	F14	F16	F18	F19	F20	F30
Metabolites	Relative LC-MS peak intensity
Fumagillin		58.2	100	60.2	41.8	nd	nd	nd
Trypacidin		27.2	100	100	81.7	24.5	70.0	nd
Fumigaclavine A		nd	46.3	100	47.2	26.0	nd	nd
Helvolic acid		nd	56.5	83.1	100	77.2	nd	nd
Monomethylsulochrin		nd	11.1	89.2	100	92.3	98.5	nd
Questin		1.3	5.2	57.4	100	81.8	100	1.7
Verruculogen		12.6	53.6	53.6	88.9	97.5	100	11.5
Fumiquinazoline C		nd	18.2	49.3	48.5	61.3	100	nd
Tryptoquivaline F		9.8	5.7	23.8	29.5	44.0	100	43.5
cell viability (%)	95.2	34.2	2.1	2.8	0.2	34.3	22.6	88.6

Fractionation of bulk culture extracts using flash chromatography. To measure their toxicity, each 5 ml fraction was dried and solubilised in 500 µl in PBS-DMSO 40% and then diluted 10 fold in PBS-DMSO 12%. The final concentration of DMSO in the assay medium was 1.2%. The cells were exposed for 24 h before measuring the cell viability as described in the Materials and Methods. The effect of 1.2% DMSO was used as negative control. For each metabolite, the figures are arbitrary units indicating its elution profile with 100 corresponding to the maximum normalized level measured by the LC-MS sensor. nd means not detectable.