Fig. 1.

Schematic outline of the DAzLE protocol. This scheme contains three steps: (i) construction and primary screening of a nonamplified cDNA library, (ii) differential hybridization analysis of cDNA clones, and (iii) clone analysis by sequencing and reverse Northern blot analysis. Differential hybridization analysis of the cDNA library was first performed with screening of random clones on duplicated nylon membranes. Positive clones that are isolated from primary screening were confirmed by reverse Northern blot analysis. Finally, the Northern blot analysis was used to confirm the differential expression of cDNA clones.