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. 2003 Dec 30;101(2):647–652. doi: 10.1073/pnas.0305145101

Fig. 4.

Fig. 4.

Analysis of differentially expressed genes in cortical neurons. (A) Reverse Northern blot analysis of differentially expressed genes isolated by DAzLE. Differentially expressed genes were dot blotted onto nylon membranes and hybridized with [32P]cDNA samples of rat E17 cortical neurons. The neurons were treated with KCl (50 mM), l-NAME (100 μM), KCl (50 mM) plus l-NAME (100 μM), KCl (50 μM) plus N-Arg (100 μM), KCl (50 mM) plus N-Arg (100 μM) plus l-Arg (10 mM), KCl (50 mM) plus nifedipine (5 μM), NOR3 (10 μM), or NOR3-NO– (10 μM), for 6 h. (B) Northern blot analysis of genes differentially expressed in KCl-induced cortical neurons. Northern blot analysis was performed on mRNA (5 μg) isolated from primary culture of rat E17 cortical neurons, which received the following treatments: (i) unstimulated, (ii) KCl (50 mM), (iii) KCl (50 mM) plus N-Arg (100 μM), and (iv) KCl (50 mM) plus nifedipine (5 μM). Control hybridizations were performed by using rat GAPDH cDNA.