Table 3. Frequency in human tumours and functional impairment of some experimentally induced mutants.
| Mutant | Aetiology(1) | Rank(2) | Nr of occurrence and frequency (%) in human tumours(3) | Loss of transcriptional activity(4) | Dominant negative effect(5) |
| Arg175His | AFB1 17, B[a]P 12 | 1 | 1130 (4.25) | ++ | ++ |
| Arg248Gln | Ac 18–21, B[a]P 22–24 | 2 | 855 (3.21) | ++ | ++ |
| Arg273Cys | Ac 27–28 | 5 | 650 (2.44) | ++ | − |
| Gly245Ser | Ac 14–16, AFB1 31–35 | 8 | 423 (1.60) | ++ | ++ |
| His179Tyr | B[a]P 14 | 19 | 104 (0.39) | ++ | ++ |
| Pro151Ser | AFB1 12 | 27 | 96 (0.36) | ++ | + |
| Pro278Leu | Ac 29–30 | 37 | 79 (0.30) | ++ | + |
| Gly244Asp | AFB1 27–28 | 56 | 60 (0.23) | ++ | ++ |
| Gly279Glu | B[a]P 31 | 69 | 48 (0.18) | ++ | ++ |
| Cys277Tyr | B[a]P 29 | 101 | 28 (0.11) | ++ | + |
| Arg267Trp | Ac 25–26 | 119 | 27 (0.10) | ++ | + |
| His179Asn | AFB1 18–20 | 128 | 23 (0.09) | ++ | ++ |
| Arg283His | Ac 31–33, AFB1 39, B[a]P 33–34 | 277 | 17 (0.06) | + | +/− |
| Ser127Pro | B[a]P 5 | 417 | 6 (0.02) | ++ | ++ |
The table shows the 14 mutants induced by acetaldehyde (Ac), B[a]P or AFB1 for which exhaustive functional data were available from Dearth et al. [12].
(1)
The number refers to the identity of the mutant in the corresponding paper: Ac, table 3 from [15]; AFB1, table 3 from [16]; B[a]P, table 2 from the present work.
(2)
According to Dearth et al. [12].
(3)
According to IARC database R14.
(4)
++: loss of transcriptional activity on the 22 p53 DBS tested; +: loss of transcriptional activity on 13 DBS only [12].
(5)
++: DNE on the 11 DBS tested; +: DNE on more than 5 DBS; +/−: DNE on 3 DBS only; −: no DNE [12].