Table 1. Acyl-CoA levels in developing castor endosperm.
Stagea | 18:1-OH CoA | 18:1 CoA | 16:0 CoA | 18:0 CoA | 18:2 CoA |
III | 4.25 (0.34) | 7.18 (0.89) | 32.25 (3.04) | 31.73 (4.36) | 7.52 (0.62) |
IV | 5.17 (0.84) | 6.59 (0.42) | 23.74 (1.88) | 31.34 (0.35 | 6.21 (0.64) |
V | 4.90 (0.22) | 7.97 (0.52) | 33.05 (3.21) | 26.65 (4.44) | 4.45 (0.31) |
VI | 8.01 (0.89) | 6.61 (0.71) | 28.89 (3.25) | 28.08 (1.23) | 7.89 (1.71) |
VII | 3.82 (0.42) | 5.17 (0.38) | 33.63 (0.40) | 31.35 (4.09) | 4.68 (0.50) |
VIII | 7.03 (0.37) | 4.23 (0.51) | 27.93 (1.49) | 26.12 (3.45) | 4.13 (0.74) |
The percentage of the total acyl-CoA peak area represented by specified acyl-CoAs are shown for castor endosperm stages during seed development. Analysis of fluorescent acyl-CoA derivatives was as in [24] and average values from three extractions and analyses for each stage are shown, together with the standard error in brackets (n = 3).
Endosperm samples were staged according to [25].