Table 1. RT–PCR parameters for SNARE elements.
| Gene | Accession number | Primer sequence | Number of cycles | BP size | Annealing temperature |
|---|---|---|---|---|---|
|
Actb |
NM_031144 |
Sense: 5′-agagggaaatcgtgcgtgaca-3′ |
33 |
202 |
59 °C |
|
|
|
Antisense: 5′-cgatagtgatgacctgaccgtca-3′ |
|
|
|
|
Rab3D |
NM_080580 |
sense: 5′-actgatggtgacaatgatgc-3′ |
37 |
340 |
59 °C |
|
|
|
antisense: 5′-acggaagtgaagaaagcaac-3′ |
|
|
|
|
Rab27b |
NM_053459 |
sense: 5′-cggagctcgagaagactaga-3′ |
37 |
225 |
60 °C |
|
|
|
antisense: 5′-ggccaggagtttaatcaggt-3′ |
|
|
|
|
Vamp2 |
012663 |
Sense: 5′-gcatctctcctaccctttca-3′ |
34 |
141 |
58 °C |
| Antisense: 5′-tttaggggtctgagggtaca-3′ |