Fig. 2.

Characterization of the interaction sites of β-catenin and the cslo α-subunit by two-hybrid experiments. (A) Using the carboxyl-terminal region of cslo α-subunit as bait, we obtained from a sensory epithelial library three positive clones, each of which represented a part of chicken β-catenin. Five β-catenin fragments were additionally tested with bait constructs to clarify the domains necessary for the interaction with the α-subunit. (B) To map the interaction site in the cslo α-subunit, we examined eight fragments in bait vectors with prey clone 7 from A. Interaction requires the S10 region of the cslo α-subunit and both the carboxyl-terminal half of the ninth armadillo repeat and the carboxyl terminus of β-catenin. (Inset) Schematic diagram of the slo channel.