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. Author manuscript; available in PMC: 2013 Feb 3.
Published in final edited form as: J Proteome Res. 2011 Dec 7;11(2):1018–1026. doi: 10.1021/pr200770v

Fig. 1.

Fig. 1

Tyrosine phosphorylation of the Kv2.1 channel is induced by pervanadate treatment. (A) After ionomycin, carbachol, or pervanadate treatment, tyrosine phosphorylation of Kv2.1 was detected only in the pervanadate-treated sample. HEK293 cells were transfected with Kv2.1 expression plasmid; cells were treated with DMSO, ionomycin, carbachol, or pervanadate, lysed and immunoprecipitated with anti-Kv2.1 mAb. The immunoprecipitation products were separated by 7.5% SDS-PAGE and immunoblotted with anti-Kv2.1 and anti-PY-20 mAbs. (B) The effect of pervanadate concentration on Kv2.1 tyrosine phosphorylation. HEK293 cells were transfected with Kv2.1 expression plasmid. Cells treated with pervanadate (0, 25, 100, or 300 μM) were lysed and immunoprecipitated with anti-Kv2.1 (top and middle) and anti-PY20 (bottom). The immunoprecipitates were separated by 7.5% SDS-PAGE and immunoblotted with anti-PY20 (top) and anti-Kv2.1 mAbs (middle and bottom).