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. Author manuscript; available in PMC: 2013 Feb 3.
Published in final edited form as: J Proteome Res. 2011 Dec 7;11(2):1018–1026. doi: 10.1021/pr200770v

Fig. 5.

Fig. 5

Effects of the constitutively active form of Src, Src-CA, on outward K+ currents in Kv2.1-expressing HEK cells using the whole-cell patch clamp technique. (A) (Left) Current-voltage relations of WT Kv2.1 channels expressed in the absence (n = 14, filled symbols) or presence of Src-CA (n = 15, open symbols). (Right) Representative whole-cell K+ currents and mean ± S.E.M. current densities from HEK cells expressing Kv2.1-WT or Kv2.1-WT and Src-CA (B) (Left) Current-voltage relations of Kv2.1-Y124F channels expressed in the absence (n = 7, filled symbols) or presence of Src-CA (n = 13, open symbols). (Right) Representative whole-cell K+ currents and mean (± S.E.M.) current densities in HEK cells expressing Kv2.1-Y124F or Kv2.1-Y124F and Src-CA. (C) (Left) Current-voltage relations of Y686F Kv2.1 channels expressed in the absence (n = 9, filled symbols) or presence of Src-CA (n = 12, open symbols). (Right) Representative whole-cell K+ currents and mean (± S.E.M.) current densities in HEK cells expressing Kv2.1-Y686F or Kv2.1-Y686F and Src-CA. (D) (Left) Current-voltage relations of Kv2.1-Y686F channels expressed in the absence (n = 20, filled symbols) or presence of Src-CA (n = 19, open symbols). (Right) Representative whole-cell K+ currents and mean (± S.E.M.) current densities in HEK cells expressing Kv2.1-Y686F or Kv2.1-Y686F and Src-CA. (*P < 0.01, **P < 0.05; Student's t-test). Current density was calculated as current amplitude at +30 mV (normalized to cell capacitance) of the K+ current evoked by sequential 10 mV voltage steps to +50 mV from a holding potential of −60 mV. Calibration: 300 pA, 100 ms.