Skip to main content
. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Hepatology. 2012 Mar;55(3):856–868. doi: 10.1002/hep.24723

Figure 4. Ionomycin-induced changes in [Ca2+]c and in SOCE are lower in CPA-treated WT cholangiocytes.

Figure 4

Cells were preincubated with cyclopiazonic acid (CPA, 100 nM, for 24 h) to achieve a moderate reduction in ER [Ca2+], then loaded with Fura-2 (5 μM), incubated in a Ca2+ free buffer in presence of EGTA (3mM) and exposed to Ionomycin (100 nM), other conditions as in Fig. 2 and 3. A) Representative traces. CPA, WT cells incubated for 24 hours with 100 nM CPA to chronically deplete the stores. B) Summary of experiments as peak increase with respect to baseline or as AUC (see Fig. 2). C) Conditions as in Fig. 3, panels C and D. CPA refers to WT cells incubated for 24 hours with 100 nM CPA to chronically deplete the stores (*p<0.01 vs, untreated cholangiocytes **p<0.001 vs untreated cholangiocytes, n=38)