Figure 2. Spatial expression of pks2.
WMISH was performed on embryos at different stages of development using a RNA digoxygenin-labeled probe. Tiles are labeled with the post-fertilization time points. (A, B) Hatched blastula; (C) Mesenchyme blastula; (D, E) Early gastrula; (F) Late gastrula. (G, H) Early prism. (A, C, D, G) Side view; (B, E, F, H) Vegetal view. Expression of pks2 is specific to skeletogenic cells (PMCs) and their precursors. The black arrows indicate the ventrolateral clusters. By the prism stage, gene expression is limited to just a few PMC descendants.