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. Author manuscript; available in PMC: 2012 Aug 1.
Published in final edited form as: Nat Struct Mol Biol. 2012 Jan 8;19(2):246–252. doi: 10.1038/nsmb.2212

Figure 3. Mre11 interacts with CDK2.

Figure 3

(a) Mre11 co-immunoprecipitates CDK2 and cyclin A. Western blot of coIPs from Mre11+/− MEFs, using α–Mre11 antibody (CoIP) or beads only (mock).

(b) CDK2 co–immunoprecipitates the MRN complex. Western blot analyses of coIPs from HeLa cells using α–HA antibody recognizing transiently expressed HA–tagged CDK2 (CoIP), or irrelevant α–GAPDH antibody control (mock).

(c) Cyclin A co–immunoprecipitates Mre11 from Mre11+/− MEFs (top). Cyclin A co–immunoprecipitates CDK2 from Mre11−/− MEFs (bottom).

(d) Mre11 does not co–immunoprecipitate CDKs other than CDK2. Western blot of co–IPs from CDK2−/− MEFs, using α–Mre11 antibody (CoIP) or beads only (mock).

(e) CoIP of Mre11 with CDK2/cyclin A requires an unperturbed Mre11 C–terminus. Western blot analyses of CoIPs using anti–HA antibody recognizing transiently expressed HA–tagged CDK2 (CoIP), or beads only control (mock). MEFs (Mre11cond/−) expressed endogenous wild–type Mre11 from the Mre11cond allele and cDNA encoding either Mre11C54 (left) or Mre11ATLD1 (right).