Table 3. Media used for culture of bacteria, fungus, Acanthamoeba from ocular samples.
| Type of sample | Culture media | Expected organisms |
|---|---|---|
| Lid margin scales | Sheep blood agar Brain heart infusion broth Sabouraud dextrose agara | Bacteria Fungi |
| Conjunctival swab | Sheep blood agar Sheep blood chocolate agar Brain heart infusion broth Sabouraud dextrose agara | Bacteria Fungi |
| Corneal scrapings | Sheep blood agar (aerobic, anaerobic) Sheep blood chocolate agar Brain heart infusion broth Sabouraud dextrose agara Thioglycollate broth Non-nutrient agar with E.coli | Bacteria (aerobic, anaerobic) Fungi Acanthamoeba |
| Aqueous/vitreous | Sheep blood agar (aerobic, anaerobic) Sheep blood chocolate agar Brain heart infusion broth Sabouraud dextrose agara Thioglycollate brotha | Bacteria (aerobic, anaerobic) Fungi |
| Corneal biopsy/ buttons | Sheep blood agar (aerobic, anaerobic) Sheep blood chocolate agar Brain heart infusion broth Sabouraud dextrose agara Non-nutrient agar with E.coli | Bacteria (aerobic, anaerobic) Fungi Acanthamoeba |
| Contact lenses | Sheep blood chocolate agar (aerobic, anaerobic) Sabouraud dextrose agara Non-nutrient agar with E.coli | Bacteria (aerobic, anaerobic) Fungi Acanthamoeba |
| Contacat lens solutions | Sheep blood chocolate agar (aerobic, anaerobic) Sabouraud dextrose agara Non-nutrient agar with E.coli | Bacteria Fungi Acanthamoeba |
| Intraocular lens/iris tissue | Sheep blood agar | Bacteria Fungi |
a
With antibiotics (gentamicin or chloramphenicol) but without cycloheximide. Potato dextrose agar may be used in addition to Sabouraud dextrose agar for better sporulation.