Fig. 4.

Sperm binding to Zp2^Mut and Zp3^Mut eggs and embryos. (A) Normal (1 to 6), Zp2^Mut (7 to 9), and Zp3^Mut (10 to 12) eggs or Zp2^Mut two-cell embryos (13 to 15) were inseminated with capacitated Acr-EGFP sperm (1 × 10⁵ ml⁻¹ of HTF), and fertilized eggs and embryos were imaged at 1, 2, and 4 hours by differential interference contrast and confocal microscopy. (B) Capacitated Acr3-EGFP sperm were incubated in the same media as in (A) and assayed by computer-assisted sperm analysis after 1, 2, 4, 6, and 24 hours. Each data point, which is an average of three independent biological samples ± SEM, reflects the percent of sperm meeting the specific criteria. (C) Ovulated eggs in cumulus from normal, Zp2^Mut, and Zp3^Mut mice were inseminated with capacitated sperm (1 × 10⁵ sperm ml⁻¹ of HTF), and fertilization was assayed by the presence of two-cell embryos after overnight incubation. The data reflect the average of five separate experiments for each genotype (a total of 66 to 92 eggs) ± SEM.