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Localization of SMARCAD1 to pericentric heterochromatin is not dependent on KAP1 levels. (A) F9 embryonic carcinoma cells and (B) F9 cells that were engineered to express low levels of KAP1/TIF1β (TIF1β-/-/rTA-f.TIF1β) were differentiated for 7 d by exposure to 1 µM retinoic acid as described by Cammas et al. representative cells stained for KAP1 (ab22553) and SMARCAD1 9 are shown, images were adjusted for brightness and contrast. DAPI bright foci mark pericentric heterochromatin.
