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. 2011 Dec 1;10(23):4119–4127. doi: 10.4161/cc.10.23.18287

Figure 1.

Figure 1

C1qbp suppresses staurosporine-induce apoptosis in fibroblasts. (A) Mouse embryonic fibroblasts (MEFs) were infected with an adenovirus encoding Myc-tagged C1qbp, or β-galactosidase (β-gal, control) and then protein gel blotted for Myc, cleaved caspase-3 and cleaved PARP following exposure to 300 nM staurosporine for 18 h. GAPDH was used to demonstrate equivalent loading. (B) TUNEL staining in the infected MEFs exposed to increasing concentrations of staurosporine for 18 h. (C) MEFs were transfected with 100 nM of control (Con) or C1qbp-specific siRNA and protein gel blotted for C1qbp, cleaved caspase-3, cleaved PARP and GAPDH following exposure to 300 nM staurosporine for 18 h. (D) TUNEL staining in the siRNA-transfected MEFs exposed to increasing concentrations of staurosporine for 18 hrs. The results shown are representative of 3 and 4 independent experiments performed in duplicate. Error bars indicate SEM and *p < 0.05 vs. β-gal or Con siRNA.