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. Author manuscript; available in PMC: 2013 Mar 15.
Published in final edited form as: Arch Biochem Biophys. 2011 Oct 7;519(2):112–117. doi: 10.1016/j.abb.2011.10.003

Fig. 3.

Fig. 3

Effects of diamide and dithiothreitol on IDH. (A) The IDH1/IDH2C56S/C242S mutant enzyme which contains IDH2 Cys-150 as the only cysteine residue was incubated with increasing concentrations of diamide prior to non-denaturing gel electrophoresis (with staining using Coomassie blue) and activity assays. (B) The diamide-treated IDH1/IDH2C56S/C242S mutant enzyme was incubated with increasing concentrations of dithiothreitol (DTT) prior to non-denaturing gel electrophoresis and activity assays. The disulfide bond form of IDH2 migrates aberrantly slowly under these conditions [38, 40], so only portions of the gels are shown.