Fig. 2. TLR3-mediated chemokine induction by HCV infection depends on viral replication.
Huh7.5 and 7.5-TLR3 cells were infected with JFH1 virus (HCV, MOI=1). Induction of RANTES and MIP-1β transcripts at indicated time points was determined by qPCR, using poly-I:C stimulation in medium as a positive control (A). Reporter assay of HCV activation of the RANTES promoter is shown in (C). “**” indicates statistical difference exists between mock- and HCV-infected 7.5-TLR3 cells with a p-value of <0.01. (B) qPCR quantification of RANTES and MIP-1β transcripts in 7.5-TLR3 cells mock-infected and infected with JFH1 virus (HCV) or UV-inactivated JFH1 virus (HCV-UV) (MOI=1) for 48 h.