Fig. 4. NF-κB controls chemokine induction in HCV-infected 7.5-TLR3 cells.

(A) qPCR quantification of the p65 NF-κB subunit binding to the indicated promoters after ChIP enrichment of chromatin from cells mock-infected or infected with JFH1 virus (MOI=0.5) for 48 h. The TFF1 promoter devoid of NF-κB binding element was used as a negative control. “**” indicates statistical differences exist between HCV/p65 antibody and mock/p65 antibody groups with a p-value of <0.01. (B) 7.5-TLR3 cells were mock-infected or infected with JFH1 virus (MOI=0.5) for 8 h, and subsequently mock-treated or treated with CAPE for 64 h prior to qPCR analysis of RANTES mRNA and HCV RNA levels.