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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Diabetologia. 2011 Dec 11;55(3):763–772. doi: 10.1007/s00125-011-2388-x

Fig. 2.

Fig. 2

Biochemical analysis of lipid metabolism in skeletal muscle of male mice. Mixed skeletal muscle was isolated (gastrocnemius and soleus) from overnight-fasted 5 to 7 months old male mice (white bars, Cc2+/+; black bars, Cc2−/− all panels). a mRNA expression of genes involved in fatty acid uptake and oxidation was determined by semi-quantitative real-time PCR and normalised to Gapdh. b FATP1 abundance by western blot analysis, using α-FATP1 antibody to immunoblot (Ib) and α-actin antibody to reprobe (reIb) and normalise against the amount of protein loaded. c Ex vivo palmitate oxidation. d triacylglycerol content. Values are expressed as mean±SEM; n=4–8 per group; *p<0.05 vs Cc2+/+