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. Author manuscript; available in PMC: 2012 Feb 5.
Published in final edited form as: Hum Mol Genet. 2009 Jun 23;18(18):3452–3461. doi: 10.1093/hmg/ddp289

Figure 1. ELISA of fH-Ile62 and fH-Val62 binding to C3b.

Figure 1

(a) Interaction between serial dilutions of purified fH-Ile62 (open circles) or fH-Val62 (filled circles) with C3b deposited in 96-well plates is expressed as Abs492. Means ± S.D. of three independent experiments are shown. Inset panel shows the double reciprocal plot of the fH-Ile62 (open circles) and fH-Val62 (filled circles) C3b-binding curves. Multiple linear regression analysis revealed significant differences between Val62 and Ile62 binding to C3b (P<0.0001).

(b) SDS-PAGE illustrating the fH-Ile62 and fH-Val62 purified from the plasma of homozygote carriers as described in Materials and Methods and then gel filtered to remove aggregates.