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. Author manuscript; available in PMC: 2012 Feb 5.
Published in final edited form as: Blood. 2009 Dec 3;115(5):995–1005. doi: 10.1182/blood-2009-03-212670

Figure 5. Expression of Bmf and Bad in (pre)-malignant Eμ-myc and wt B lymphocytes.

Figure 5

Wt and Eμ-myc FACS-sorted pre-B cells from the bone marrow and IgM+ B cells from the spleens of 4-week-old mice were analyzed for expression of (A) c-Myc, Bmf or Bad. Representative blots from two independent cell sorts are shown. Membranes were reprobed using anti-MAPK, anti-actin as a loading control. (B) Representative immunoblots assessing expression of Bmf and Bad in Eμ-myc-driven tumors. Membranes were reprobed using anti-GAPDH antibody as a loading control. (C) Representative immunoblots assessing LOH in Eμ-myc-driven tumors derived from bmf+/− or bad+/− animals. Membranes were reprobed using anti-actin antibody as a loading control.