Skip to main content
. 2012 Jan 4;32(1):223–228. doi: 10.1523/JNEUROSCI.2596-11.2012

Figure 4.

Figure 4.

Expression of dominant-negative N-cadherin rescues apical retraction in slit1b morphant RGCs. A–H, Confocal images from control morpholino-injected (CoMO) embryos expressing Hsp70:mCherryCAAX (A, B) or Hsp70:NcadhΔEC-mCherry (C, D); confocal images from slit1b morphants expressing Hsp70:mCherryCAAX (E, F) or Hsp70:NcadhΔEC-mCherry (G, H) at 48 hpf. Zn5 (pseudocolored in green) labels the ganglion cells. Boxed regions in A, C, E, and G are enlarged in B, D, F, and H, respectively. Arrowheads in F indicate the un-retracted apical process of an Hsp70:mCherryCAAX-expressing RGC (defined as cells that are Zn5+) in a slit1b morphant; arrowheads in B, F, and H indicate mCherry-positive RGCs with retracted apical processes. Scale bars, 20 μm. I, Percentage of mCherry-positive RGCs with un-retracted apical processes at 48 hpf (n = 44 Hsp70:mCherryCAAX-expressing cells from control morpholino-injected embryos; n = 61 Hsp70:NcadhΔEC-mCherry-expressing cells from control morpholino-injected embryos; n = 38 Hsp70:mCherryCAAX-expressing cells from slit1b morphants; n = 41 Hsp70:NcadhΔEC-mCherry-expressing cells from slit1b morphants). Error bars indicate SEs (**p < 0.01; ***p < 0.001).