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. Author manuscript; available in PMC: 2012 Feb 5.
Published in final edited form as: Cancer Res. 2010 Aug 24;70(17):6988–6998. doi: 10.1158/0008-5472.CAN-10-0242

Figure 2. PN-1 is targeted by MMP-9 in vivo.

Figure 2

A) Tissue lysates of indicated organs from C57/B6 WT (+/+) or MMP-9 KO (−/−) mice were resolved by SDS-PAGE gel and blotted with anti-mouse PN-1 or anti-β-actin antibody (i and ii). 20ng recombinant mouse PN-1 protein (rPN-1) was a control. Please note β-actin is not present in heart or muscle. Panel iii) shows Panel i) after longer exposure. A PN-1 degradation fragment was now apparent in the lysates from the seminal vesicles and the prostate. B) Primary bone marrow derived cells (BMDC) were grown from C57/B6 WT (+/+) or MMP-9 KO (−/−) mice, serum free conditioned media was subjected to immunoblot with anti-MMP-9 or anti-PN-1 antibody. C) Immunohistochemistry showing the expression of PN-1 (green) in seminal vesicles, pancreas and prostate from WT or MMP-9 KO mice. Cell nuclei were stained by Hoechst (blue).