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. Author manuscript; available in PMC: 2012 May 1.
Published in final edited form as: Lab Invest. 2011 Oct 3;92(2):256–264. doi: 10.1038/labinvest.2011.148

Figure 3.

Figure 3

Activation of Wnt-signaling down-regulates Olfm-1 expression and reverses the suppressive effect of Olfm-1 on spheroids attachment. (A) Upper panel shows the activation of Wnt-signaling pathway by Wnt3a-conditioned medium (1:2 ratio) or LiCl (40mM) resulted in an elevated total β-catenin but reduced Olfm-1 expression. No change in the expression of GSK-3β and β-actin were found. The lower panel depicts the changes of active-β-catenin and Olfm-1 in the OE-E6/E7 cells treated 24h with Wnt3a-CM. (B) Wnt3a-CM and LiCl stimulate JAr spheroids attachment onto OE-E6/E7 monolayer after 1 hr coculture (upper panel). The increased JAr spheroids attachment by Wnt3a-CM can be nullified by co-treatment of Wnt3a-CM with rhOlfm-1 or rhDkk-1 (lower graph). a, b, c and d denotes significant different from each other at p<0.05.