Table 3.
Primer sequences, source and annealing temperatures. Optimized annealing temperatures in bold.
| Gene | Forward | Sequence | Reference | Reverse | Sequence | Reference | Annealing Temperature |
|---|---|---|---|---|---|---|---|
| COI | 1718 | 5-ggA ggA TTT ggA AAT TgA TTA gTT CC-3 | Simon et al. (1994) | 2568 | 5-gCT ACA ACA TAA TAA gTA TCA Tg-3 | Simon et al. (1994) | 44-50, 48°C |
| COI | 1751 | 5 -gAg CTC CTg ATA TAg CTT TTC C-3 | Simon et al. (1994) | 2568 | 5-gCT ACA ACA TAA TAA gTA TCA Tg-3 | Simon et al. (1994) | 44-50, 48°C |
| COI | PMT1 | 5-GGT CAA CAA ATC ATA AAG ATA TTG G-3 | Folmer et al. (1994) | 2568 | 5-gCT ACA ACA TAA TAA gTA TCA Tg-3 | Simon et al. (1994) | 44-50, 45°C |
| COI | 1490-ONO | 5-CW ACA AAY CAT ARR GAT ATT GG-3 | Simon et al. (1994) | 2568 | 5-gCT ACA ACA TAA TAA gTA TCA Tg-3 | Simon et al. (1994) | 44-50, 45°C |
| COI | 2309 | 5-TTT ATg CTA TAg TTg gAA TTg g-3 | Simon et al. (1994) | 2776 | 5-ggA TAA TCA gAA TAN CgN CgA gg-3 | Simon et al. (1994) | 44-50, 48°C |
| 28srDNA | ZX1 | 5-ACC CGC TGA ATT TAA GCA TAT-3 | Mallatt and Sullivan (1998) | ZR2 | 5-CCG AAG TTT CCC TCA GGA TAG C-3 | Mallatt and Sullivan (1998) | 50-60, 55°C |
| 28srDNA | 28sOCS | 5-CGT GAA ACT GCT CAG AGG-3 | Miller et al. (2010) | 28sC | 5-GGC GAA AGA CTA ATC GAA CC-3 | Miller et al. (2010) | 50-60, 55°C |
| Histone 3 | H3af | 5-ATG GCT CGT ACC AAG CAG ACV GC-3 | Colgan et al. (1998) | H3ar | 5-ATA TCC TTR GGC ATR ATR GTG AC-3 | Colgan et al. (1998) | 48-55, 50°C |
| Histone 3 | H3nf | 5-ATG GCT CGT ACC AAG CAG AC-3 | Colgan et al. (1998) | H3nr | 5-ATR TCC TTG GGC ATG ATT GTT AC-3 | Colgan et al. (1998) | 48-55, 50°C |