Fig. 2.
Mitoxantrone prolongs survival in CPXV-infected C57Bl/6 mice. Female C57Bl/6 mice were inoculated intraperitoneally with 1 × 106 PFU CPXV. One day post-inoculation, animals (n = 20) received 0.5 mL of MXN at the indicated dosage or saline solution intraperitoneally. Data are a compilation of two independent experiments. Kaplan–Meier survival curves plot the survival times by dose. The lower dose of MXN significantly improved the survival time of infected animals, with the median day-of-death (MDD) in infected animals increasing from day 7 to day 9 (p = 0.0015; log-rank test), while 0.5 mg/kg of MXN improved the MDD to 12.5 (p = 0.0005; log-rank test). All animal procedures were approved by the NIAID Animal Care and Use Committee and adhered to National Institutes of Health policies.