. Author manuscript; available in PMC: 2012 Feb 6.

Published in final edited form as: Mol Psychiatry. 2008 May 27;15(2):185–203. doi: 10.1038/mp.2008.53

Table 2.

Phospho-proteins identified by MALDI-TOF-TOF

Spot nos.	Protein ID (PMF)	Accession nos.	M_r (kDa)	pI	Peptides	MASCOT score		CI (%)	Protein quantitation
Spot nos.	Protein ID (PMF)	Accession nos.	M_r (kDa)	pI	Peptides	Target	Decoy	CI (%)	T-test (P-value)	Average ratio
Structural
204	Actin, β	Q96HG5	40.98	5.5	21	243	41	100	0.019	−2.08
205	ACTG1 protein (γ-actin)	P63261	41.76	5.3	18	185	49	100	0.995	1.21
183	Actin, cytoplasmic 1	P60709	41.71	5.3	20	72	43	100	0.315	−1.30
191	Actin, cytoplasmic 1	P60709	41.7	5.3	23	116	46	100	0.122	5.88
190	Actin, cytoplasmic 1	P60709	41.7	5.3	21	96	35	100	0.916	−1.56
186	Actin, cytoplasmic 1	P60709	41.7	5.3	16	102	39	100	0.315	−1.30
193	Actin, cytoplasmic 1	P60709	41.7	5.3	16	66	37	100	0.495	−1.04
111	Neurofilament, light polypeptide 68 kDa	P07196	61.74	4.7	16	66	39	100	0.032	−3.57
7	Neurofilament triplet M protein 160 kDa	P07197	102.38	4.9	25	91	38	100	0.728	−1.58
Cell signaling
274	GABA_A receptor-associated protein 1 (GEC1)	Q9H0R8	14.03	8.7	5	55	39	100	N/A	COC/0
315	Rab GDP dissociation inhibitor-α	P31150	50.55	5	17	54	41	100	0.0008	−1.54
119	Rab GDP dissociation inhibitor-α	P31150	50.55	5	17	69	38	100	0.192	−1.72
256	14-3-3 γ-protein	P61981	28.36	4.7	13	92	46	99.99	0.057	4.79
255	14-3-3 γ-protein	P61981	28.36	4.7	11	86	37	99.99	N/A	COC/0
264	ρ-GDP dissociation inhibitor-α	P52565	23.2	5.0	6	69	40	100	0.241	2.22
Metabolism
286	Glutathione S-transferase pi—Macaca mulatta	Q28514	23.42	5.9	11	107	46	100	N/A	COC/0
267	Glutathione S-transferase pi—human	P09211	23.37	5.4	7	54	39	100	N/A	COC/0
293	Peroxiredoxin 2 isoform b	P32119	159.79	6.1	10	141	44	100	0.014	−3.37
219	Brain-type aldolase	P09972	39.42	6.4	17	92	46	100	N/A	COC/0
115	Brain-type aldolase	P09972	39.43	6.4	16	93	44	100	N/A	COC/0
245	Dimethylarginine dimethylaminohydrolase 2	Q5SRR8	29.63	5.7	8	59	38	100	N/A	COC/0
172	Guanine deaminase	Q9Y2T3	52.8	5.6	22	95	44	100	N/A	0/CTR
217	Fructose-bisphosphate aldolase C	P09972	39.43	6.4	15	82	39	100	0.596	−2.38
Mitochondrial
166	ATP-synthase β-chain, mitochondrial precursor	P06576	56.52	5.3	20	103	51	100	0.569	−3.69
164	ATP-synthase β-chain, mitochondrial precursor	P06576	56.52	5.3	15	86	41	100	0.165	−1.76
325	Ubiquitin C-terminal hydroxylase 12	O75317	24.73	5.3	12	120	44	100	0.355	−1.19
Miscellaneous
232	Crystallin, μ	Q14894	33.75	5.1	8	140	43	100	0.0012	1.68
173	KIAA1258 protein	Q9ULG2	53.4	5.5	17	91	41	100	0.444	−1.15
214	GLUL protein	P15104	42.11	6.4	15	55		100	0.351	−1.44
197	Creatine kinase B-type	P12277	42.59	5.3	25	149	39	100	0.187	−2.11
196	Creatine kinase, brain	Q6FG40	42.61	5.3	14	113	43	100	0.753	1.43
114	Heat-shock 60 kD protein 1	P10809	61.01	5.7	25	142	46	100	0.192	−1.72
331	60 kDa heat-shock protein, mitochondrial precursor (Hsp60)	P10809	61.01	5.7	17	66	35	100	0.730	1.26
101	Heat-shock 70 kDa protein 8 isoform 1	Q53GZ6	70.85	5.4	28	118	53	100	0.249	−1.48

Abbreviations: ACTG1, γ-actin 1; CI, confidence interval; GABA_A, γ-aminobutyric-acid type A; nos., numbers; pI, isoelectric points; PMF, peptide mass fingerprint.

Statistically significant differences in protein abundance between the groups are shaded. MASCOT score is shown for the searches against the target and also the decoy databases. Average ratios are provided as cocaine:control. Positive and negative ratios indicate that the protein abundance is greater in the cocaine group and control group, respectively. COC/0 refers to proteins present in three or four cocaine subjects and none of the control subjects. 0/CTR refers to proteins present in three or four control subjects and none of the cocaine subjects.