Table 3.

Phospho-proteins identified by amino acid sequencing using MALDI-TOF-MS/MS

Spot #	Protein ID (MSMS)	Accession #	Peptide sequence	Precursor mass	Mass error (ppm)	MASCOT score	Confidence interval (%)
101	Heat shock 70 kDa protein 8 isoform 1	Q53GZ6	FEELNADLFR	1253.6162	0	29	97.95
			TVTNAVVTVPAYFNDSQR	1981.9982	0	35	99.49
114	Heat shock 60 kD protein 1	P10809	AAVEEGIVLGGGCALLR	1684.9053	0	85	100
			LVQDVANNTNEEAGDGTTTATVLAR	2560.2588	4	29	98.49
166	ATP synthase beta chain, mitochondrial precursor	P06576	AIAELGIYPAVDPLDSTSR	1988.0354	1	42	99.91
172	Guanine deaminase	Q9Y2T3	FQNIDFAEEVYTR	1631.7726	2	48	99.98
196	Brain creatine kinase	Q6FG40	DLFDPIIEDR	1232.6084	6	38	99.66
			VLTPELYAELR	1303.7258	0	34	99.26
197	Brain creatine kinase	P12277	VLTPELYAELR	1303.7256	0	54	99.98
			LAVEALSSLDGDLAGR	1586.8346	2	33	98.69
204	Actin, beta	Q96HG5	QEYDESGPSIVHR	1516.6962	4	73	100
			SYELPDGQVITIGNER	1790.8821	5	64	99.99
			VAPEEHPVLLTEAPLNPK	1954.0564	4	49	99.96
205	ACTG1 protein	P63261	QEYDESGPSIVHR	1516.6975	3	48	99.97
			SYELPDGQVITIGNER	1790.889	2	72	100
232	Crystallin, mu	Q14894	LVTFYEDR	1042.5265	6	35	99.31
			VPAFLSAAEVEEHLR	1667.8766	1	97	100
255	14-3-3 gamma protein	P61981	NVTELNEPLSNEER	1643.7914	3	57	100
264	Rho GDI alpha	P52565	SIQEIQELDKDDESLR	1917.9427	1	35	99.17
267	Glutathione S-transferase pi Human	P09211	PPYTVVYFPVR	1337.7222	2	66	100
286	Glutathione S-transferase pi macaca mulatta	Q28514	PPYTVVYFPVR	1337.7238	1	31	98.63
			FQDGDLTLYQSNTFLR	1917.9314	1	29	97.88
293	Peroxiredoxin 2 isoform b	P32119	QITVNDLPVGR	1211.6733	1	49	99.97
			KEGGLGPLNIPLLADVTR	1863.067	2	29	97.78
325	Ubiquitin carboxyl-terminal esterase L1	O75317	NEAIQAAHDAVAQEGQCR	1967.8951	3	101	100