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. Author manuscript; available in PMC: 2012 Feb 6.
Published in final edited form as: J Alzheimers Dis. 2010;22(2):683–984. doi: 10.3233/JAD-2010-100816

Figure 4. Aβ40 and Aβ42 levels vary depending on cell density in mouse primary cortical neurons.

Figure 4

Mouse primary cortical neurons were seeded on poly-D-lysine/laminin-coated 24-well plates and harvested after 48h. Cell lysates were applied to BCA assay to calculate protein concentration. Conditioned media were applied to ELISA to calculate Aβ40 and Aβ42 levels, which were then normalized to protein concentration. The values of Aβ40, Aβ42, total Aβ, and the Aβ42:Aβ40 ratios from high seeding densities were compared to those from 200K. A-D At higher seeding densities (300K-600K), the levels of Aβ40 (A), Aβ42 (B), Aβ(40+42) (C), and the ratios of Aβ42:Aβ40 (D) significantly decreased compared to those at 200K (n=3 for each treatment group; mean ± S.E.; *p<0.05; **p<0.01).