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. Author manuscript; available in PMC: 2012 Feb 6.
Published in final edited form as: J Alzheimers Dis. 2010;22(2):683–984. doi: 10.3233/JAD-2010-100816

Figure 6. APP proteolytic processing varies depending on cell density in CHO-APP751 cells.

Figure 6

A-D. CHO-APP751 cells were seeded on 6-well plates with different densities. Cell lysates were collected after 48h and applied to Western blotting analysis. The levels of full-length APP, APP-CTFs (C83 and C99), and their ratios to APP from higher seeding densities were compared to those at 150K. At higher cell densities (200K-400K), full-length APP levels significantly decreased versus those at 150K (A-B). At higher seeding densities, the levels of C83 and C99, as well as their ratios to full-length APP decreased versus 150K (C-D). E. The ratios of Aβ(40+42):APP-C99 were obtained by normalizing the levels of Aβ(40+42) to APP-C99 levels from the same samples. The ratios of Aβ(40+42): APP-C99 initially increased with higher seeding densities (versus 150K), but then began to decrease with seeding densities > 250K (n=3 for each treatment group; mean ± S.E.; *p<0.05; **p<0.01).